一、 人白介素33 ELISA 試劑盒的產(chǎn)品簡述：

       ELISA 品  牌： USCN

       ELISA 貨  號：E91980Hu

       ELISA英文名：human IL33 ELISA Kit

       ELISA中文名：人白介素33,IL33 ELISA試劑盒

二、 human IL33 ELISA Kit的技術(shù)參數(shù)：
      1. 檢測范圍： 

      2. 靈  敏  度：

      3. 適用標(biāo)本：

      4.保存條件：2-8℃

       5. 有 效  期： 6個月

三、人IL33 ELISAKit的產(chǎn)品用途：
      產(chǎn)品用途：酶聯(lián)免疫實驗(ELISA實驗)

      備     注：僅供參考，請以隨貨紙質(zhì)說明書為準(zhǔn)，更多產(chǎn)品如抗體(antibody)；蛋白(protein)；鱟試劑；內(nèi)毒素工作標(biāo)準(zhǔn)品；內(nèi)毒素指示劑；無熱原檢查用水；無熱原吸咀；紫外燈；色譜儀；色譜柱等更詳細(xì)信息請登錄上海武昊公司網(wǎng)站禁發(fā)網(wǎng)址wuhaosh禁發(fā)網(wǎng)址 或致電咨詢

    
 ELISA實驗前，血清、血漿、組織、細(xì)胞培養(yǎng)上清等樣品的采集及處理

 Serum - Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4oC before centrifugation for 20 minutes at approximately 1000×g. Assay freshly prepared serum immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at  000×g at 2 - 8oC within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Tissue homogenates  - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS(0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in 5-10 mL of PBS with a glass homogenizer on ice(Micro Tissue Grinders woks, too). The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 5 minutes at 5000×g. Remove the supernate and assay immediately or aliquot and store at ≤-20oC.

Cell culture supernates    - Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.