一、大鼠PPARd ELISA 試劑盒的產品簡述：

       ELISA 品  牌：USCN

       ELISA 貨  號：072041r 

       ELISA英文名：Canine TNFa ELISA Kit

       ELISA中文名：大鼠過氧化物酶體增殖物激活受體δ，PPARd ELISAkit

二、  大鼠過氧化物酶體增殖物激活受體δELISAkit的技術參數：

      1. 檢測范圍： 

      2. 靈 敏 度：

      3. 適用標本：

      4. 保存條件：2-8℃

       5. 有 效 期：6個月

三、rat PPARd ELISA 試劑盒的產品用途：

      產品用途：酶聯免疫實驗(ELISA實驗)

           備     注：僅供參考，請以隨貨紙質說明書為準，更多產品如抗體(antibody)；蛋白(protein)；鱟試劑；內毒素工作標準品；內毒素指示劑；無熱原檢查用水；無熱原吸咀；紫外燈；色譜儀；色譜柱等更詳細信息請登錄上海武昊公司網站禁發網址wuhaosh禁發網址 或致電咨詢。

ELISA實驗前，血清、血漿、組織、細胞培養上清等樣品的采集及處理 

 Serum- Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at 4oC before centrifugation for 20 minutes at approximately 1000×g. Assay freshly prepared serum immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at  000×g at 2 - 8oC within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.

Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed in ice-cold PBS(0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weighed before homogenization. Minced the tissues to small pieces and homogenized them in 5-10 mL of PBS with a glass homogenizer on ice(Micro Tissue Grinders woks, too). The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifugated for 5 minutes at 5000×g. Remove the supernate and assay immediately or aliquot and store at ≤-20oC.

Cell culture supernates - Centrifuge samples for 20 minutes at 1000×g. Remove particulates and assay immediately or store samples in aliquot at -20oC or -80oC for later use. Avoid repeated freeze/thaw cycles.